Compressed gases expose users to both chemical and physical hazards. Gases contained within cylinders can be toxic, flammable, oxidizing, corrosive, inert, or a combination of these hazards. Because the pressurized chemical is released in gaseous form, a leak from the cylinder, regulator, or any part of the system used to deliver the gas can quickly contaminate a large area. Therefore, it is necessary to be familiar with the chemical hazards of the gas and possibility of asphyxiation. In addition to the chemical hazards, there are hazards from the gas pressure and the physical weight of the cylinder. A gas cylinder falling over can break chemical containers and crush feet. The cylinder can itself become a dangerous propelled object if its valve is broken off. Appropriate care in the handling and storage of compressed gas cylinders is essential and information is provided below.

As of July 1, 2025, SJ Smith is currently the gas cylinder vendor on campus. If you have any questions or concerns regarding gas cylinders, please contact Mike Prince, at mprince@sjsmith.com or the Division of Research Safety.

Hazard Categories for Compressed Gases

Corrosive

Gases that corrode material or tissue with which they come in contact, or do so in the presence of water, are classified as corrosive.

Flammable

A gas at normal atmospheric temperature and pressure that can be ignited and burned when mixed with the proper proportions of air, oxygen, or other oxidizers is considered flammable. Changes in temperature, pressure, or oxidant concentration may cause the flammability range to vary considerably.

Inert

Gases that do not react with other materials at standard temperature and pressure are classified as inert. They are colorless, odorless, nonflammable, and nontoxic. The primary hazards of these gases are the high pressure and potential for asphyxiation. These gases are often stored at pressures exceeding 2,000 psi. They can displace the amount of oxygen necessary to support life when released in a confined place. Use of adequate ventilation and monitoring the oxygen content in confined places will minimize the danger of asphyxiation.

Oxidizer

Gases that do not burn but will support combustion are classified as oxidants. Oxidizing gases can react violently with organic chemicals or material.

Toxic

Gases that may produce lethal or other harmful effects on humans are classified as toxic. The degree of toxicity and the effects will vary depending on the gas. The Safety Data Sheet should be consulted to determine the toxicity.

SDS (safety data sheets) for compressed gases can be found on the SJ Smith database https://sjsmith.com/resources/sds-database-literature.  If you are having trouble locating a SDS, contact SJ Smith or the supplier of the gas.

Types of Cylinders

There are two types of gas cylinders used on campus.

1. Refillable Cylinders–These are provided as a rental from a gas vendor and must be returned to them when they are empty or the gas is no longer in use. This is the recommended practice for managing gas cylinders.
2. Non-refillable Cylinders–Including lecture bottles, these are purchased from the gas vendor; the university is responsible for disposing of them properly. These cylinders are not rented; disposal costs can range from $0 (non-hazardous gases only) to over $1000 even if the container is empty. The primary reason for purchasing non-refillable gas cylinders is the size. These cylinders can be small (ca. 12-15 inches long and 2 inches in diameter) and conveniently portable. However, most gas companies now have small cylinders for rent. 

  Disposable Lecture Bottle

The Division of Research Safety encourages all researchers to rent refillable gas cylinders.

Most gas vendors are able to provide a complete line of small quantity gases for rent in refillable, returnable cylinders which are generally about 20 inches long and 4 inches in diameter. These refillable, returnable cylinders are as portable as lecture bottles and almost always cost less than the same product delivered in a lecture bottle. The cylinders contain more product than lecture bottles and may be returned to the gas vendor for free even if they are not empty.

   Small Refillable Gas Cylinder

Storing Gas Cylinders

The amount of hazardous compressed gases permitted within a laboratory work area is regulated by NFPA 45 as outlined in the table below. 

In addition to the maximum quantities listed in the table above, the number of lecture bottle cylinders is limited to 25.

1. Always secure gas cylinders and lecture bottles upright (with valve end up) to a wall, bench top, cylinder rack, or post unless the cylinder is specifically designed to be stored otherwise. Specially designed cylinder clamps can be purchased for securing a cylinder against a bench top. Gas cylinders must not be stored on gas carts. Do not strap cylinders to other cylinders.
2. Cylinders must be strapped or chained at, or slightly above, the midpoint. The strap must be tight around the cylinder to prevent it from tipping or moving.                                                                                           
   
3. Cylinders should be stored in a well-ventilated area away from sparks, flames, or any source of heat or ignition.
4. Cylinders should not be exposed to conditions that may cause corrosion.
5. Never store gas cylinders in a location that could block exit routes.
6. Cylinders may be stored outside on a slab if they are protected from the direct rays of the sun. Do not expose cylinders to temperatures above 125 ºF.
7. Mark empty cylinders with an “empty” label.

Transporting Gas Cylinders

1. Always use a suitable cylinder cart for transporting cylinders, with the cylinder securely chained or strapped to the cart. Do not roll or drag a cylinder to move it or allow cylinders to strike each other or any other surface violently. -To secure the cylinder to a cart, first move the cart within two feet of the cylinder and then slowly walk the cylinder to the cart. A cart should be used to transport the cylinder short as well as long distances.
2. Protective valve caps must be secured when moving cylinders. Do not lift or move the cylinder by the cap.

General Use

1. Caps used for valve protection should be kept on the cylinder except when the cylinder is in use. A cylinder's cap should be screwed on tightly.
2. If a cylinder or valve is noticeably corroded, the gas vendor should be contacted for instructions. Any other damage that might impair the integrity of the cylinder should be called to the attention of the gas vendor before the cylinder is returned.
3. Transferring compressed gases from one commercial cylinder to another is not permitted.
4. Do not use compressed gas cylinders for any purpose other than for transporting and supplying gas.
5. Never tamper with, repair, or alter cylinders, regulators, or any pressure-relief devices. Return defective cylinders to the gas vendor immediately.
6. Do not attempt to remove a stuck cylinder cap by using a lever in the cap ports. The lever may accidentally open the valve when the cap turns.
7. Do not place cylinders where they might become part of an electric circuit or allow them to come into contact with an electrically energized system.
8. Use pressure regulators that are equipped with pressure relief devices.
9. Users must be properly trained in using gas cylinders by someone with experience.
10. If a gas cabinet is used, label the outside of the cabinet with the contents.
11. Label all piping that contains gases. NFPA 55 identifies the following points as requiring markings:

• At each critical process control valve
• At wall, floor, or ceiling penetrations
• At each change of direction
• At a minimum of every 20 ft. or fraction thereof throughout the piping run.

Regulators

Single Stage Regulator [1]

Two-Stage Regulator [2]

Different gases and cylinder sizes have different requirements for regulators. The cylinder connection can be male or female and be composed of different materials. A CGA number printed on the gas cylinder and the regulator is used to find the correct match. Below are a few examples of different connectors and their CGA numbers.

For flammable gases, a left-handed thread is used. This is often indicated by a notch or line on the adaptor. The diaphragm within the regulator can be made from different materials. Depending on the gas used, some materials are preferred or even required. Two common materials include elastomers (e.g., neoprene) and stainless steel. The diaphragms made of elastomers can introduce contaminates to the system as they can absorb moisture or contaminates from the air. To maintain purity it is recommended to use a stainless steel diaphragm whenever possible.

Regulators do not last forever. Depending on the type of gas and frequency of use, regulators can become compromised. Contact SJ Smith to determine if the regulator you are using is safe and review this guidance for regulator maintenance. 

Never use a connection adapter to mount the wrong regulator on a cylinder. The CGA connections must match between the cylinder outlet valve and the regulator. The CGA connection depends on the type of gas and also the size of the cylinder. Modifying connections can lead to a serious risk using the cylinder.

Teflon tape should not be used at on the CGA connection. This can cause leaks, connection to fail, or contamination of the regulator. The CGA connection is designed so no additional materials are needed to improve the seal. Do not lubricate fittings. Lubricants can react with certain gases or introduce impurities to the system that can damage connections and the regulator. Carefully inspect the CGA connection for damage before using the gas. If there is any damage, contact SJ Smith.

If you notice one of the pressure gauges not working, remove it from service. Likely the gauge has been blown by an over-pressurization event causing the bourdon tube to be compromised. It is necessary to investigate how the regulator was damaged in order to prevent future incidents.

Consult with the gas supply company for questions about the correct type of regulator and materials for the gas being used.

Guidelines For Specific Hazards

Flammable Gas Safety

In addition to the above guidelines, the following measures should be taken when handling flammable gases:

Gas Cabinets for Pyrophoric Gases

Brief Storage Guide Based On Specific Hazards