Compressed gases expose users to both chemical and physical hazards. Gases contained within cylinders can be toxic, flammable, oxidizing, corrosive, inert, or a combination of these hazards. Because the pressurized chemical is released in gaseous form, a leak from the cylinder, regulator, or any part of the system used to deliver the gas can quickly contaminate a large area. Therefore, it is necessary to be familiar with the chemical hazards of the gas and possibility of asphyxiation. In addition to the chemical hazards, there are hazards from the gas pressure and the physical weight of the cylinder. A gas cylinder falling over can break chemical containers and crush feet. The cylinder can itself become a dangerous propelled object if its valve is broken off. Appropriate care in the handling and storage of compressed gas cylinders is essential and information is provided below.

Airgas is currently the gas cylinder vender on campus. If you have any questions or concerns regarding gas cylinders, please contact the Airgas University Specialist, Tyler McKenzie, at or the Division of Research Safety.

Hazard Categories for Compressed Gases


Gases that corrode material or tissue with which they come in contact, or do so in the presence of water, are classified as corrosive.


A gas at normal atmospheric temperature and pressure that can be ignited and burned when mixed with the proper proportions of air, oxygen, or other oxidizers is considered flammable. Changes in temperature, pressure, or oxidant concentration may cause the flammability range to vary considerably.


Gases that do not react with other materials at standard temperature and pressure are classified as inert. They are colorless, odorless, nonflammable, and nontoxic. The primary hazards of these gases are the high pressure and potential for asphyxiation. These gases are often stored at pressures exceeding 2,000 psi. They can displace the amount of oxygen necessary to support life when released in a confined place. Use of adequate ventilation and monitoring the oxygen content in confined places will minimize the danger of asphyxiation.


Gases that do not burn but will support combustion are classified as oxidants. Oxidizing gases can react violently with organic chemicals or material.


Gases that may produce lethal or other harmful effects on humans are classified as toxic. The degree of toxicity and the effects will vary depending on the gas. The Safety Data Sheet should be consulted to determine the toxicity.

SDS (safety data sheets) for compressed gases can be found on the Airgas website  If you are having trouble locating a SDS, contact Airgas or the supplier of the gas.

Types of Cylinders

There are two types of gas cylinders used on campus.

  1. Refillable Cylinders–These are provided as a rental from a gas vendor and must be returned to them when they are empty or the gas is no longer in use. This is the recommended practice for managing gas cylinders.
  2. Non-refillable Cylinders–Including lecture bottles, these are purchased from the gas vendor; the university is responsible for disposing of them properly. These cylinders are not rented; disposal costs can range from $0 (non-hazardous gases only) to over $1000 even if the container is empty. The primary reason for purchasing non-refillable gas cylinders is the size. These cylinders can be small (ca. 12-15 inches long and 2 inches in diameter) and conveniently portable. However, most gas companies now have small cylinders for rent. 

Disposable Lecture Bottle

The Division of Research Safety encourages all researchers to rent refillable gas cylinders.

Most gas vendors are able to provide a complete line of small quantity gases for rent in refillable, returnable cylinders which are generally about 20 inches long and 4 inches in diameter. These refillable, returnable cylinders are as portable as lecture bottles and almost always cost less than the same product delivered in a lecture bottle. The cylinders contain more product than lecture bottles and may be returned to the gas vendor for free even if they are not empty.

 Small Refillable Gas Cylinder

Storing Gas Cylinders

The amount of hazardous compressed gases permitted within a laboratory work area is regulated by NFPA 45 as outlined in the table below. 


Flammable Gas

Oxidizing Gas

Health Hazard 3 or 4 (NFPA)

Amount allowed per 500 ft2 

6.0 scf

6.0 scf

0.3 scf

Number of cylinders allowed per 500 ft2

3 cylinders (9"x51" cylinder)

3 cylinders (9"x51" cylinder)


Amount of gas allowed per ft2 of lab space

0.012 ft3

0.012 ft3

0.0006 ft3

In addition to the maximum quantities listed in the table above, the number of lecture bottle cylinders is limited to 25.

  1. Always secure gas cylinders and lecture bottles upright (with valve end up) to a wall, bench top, cylinder rack, or post unless the cylinder is specifically designed to be stored otherwise. Specially designed cylinder clamps can be purchased for securing a cylinder against a bench top. Gas cylinders must not be stored on gas carts. Do not strap cylinders to other cylinders.
  2. Cylinders must be strapped or chained at, or slightly above, the midpoint. The strap must be tight around the cylinder to prevent it from tipping or moving.
  3. Cylinders should be stored in a well-ventilated area away from sparks, flames, or any source of heat or ignition.
  4. Cylinders should not be exposed to conditions that may cause corrosion.
  5. Never store gas cylinders in a location that could block exit routes.
  6. Cylinders may be stored outside on a slab if they are protected from the direct rays of the sun. Do not expose cylinders to temperatures above 125 ºF.
  7. Mark empty cylinders with an “empty” label.


Transporting Gas Cylinders

  1. Always use a suitable cylinder cart for transporting cylinders, with the cylinder securely chained or strapped to the cart. Do not roll or drag a cylinder to move it or allow cylinders to strike each other or any other surface violently.                                                                                                                                                        -To secure the cylinder to a cart, first move the cart within two feet of the cylinder and then slowly walk the cylinder to the cart. A cart should be used to transport the cylinder short as well as long distances.
  2. Protective valve caps must be secured when moving cylinders. Do not lift or move the cylinder by the cap.


General Use

  1. The gas label provided by the vendor must be kept in its original condition. Install the cylinder so the label can be easily read. Label should be visible from any direction of approach. If this is not possible, label the cylinder with an additional label or sign. This is especially important in the event of an emergency.
  2. Caps used for valve protection should be kept on the cylinder except when the cylinder is in use. A cylinder's cap should be screwed on tightly.
  3. If a cylinder or valve is noticeably corroded, the gas vendor should be contacted for instructions. Any other damage that might impair the integrity of the cylinder should be called to the attention of the gas vendor before the cylinder is returned.
  4. Transferring compressed gases from one commercial cylinder to another is not permitted.
  5. Do not use compressed gas cylinders for any purpose other than for transporting and supplying gas.
  6. Never tamper with, repair, or alter cylinders, regulators, or any pressure-relief devices. Return defective cylinders to the gas vendor immediately.
  7. Do not attempt to remove a stuck cylinder cap by using a lever in the cap ports. The lever may accidentally open the valve when the cap turns.
  8. Do not place cylinders where they might become part of an electric circuit or allow them to come into contact with an electrically energized system.
  9. Use pressure regulators that are equipped with pressure relief devices.
  10. Users must be properly trained in using gas cylinders by someone with experience.
  11. If a gas cabinet is used, label the outside of the cabinet with the contents.
  12. Label all piping that contains gases. NFPA 55 identifies the following points as requiring markings:
  • At each critical process control valve
  • At wall, floor, or ceiling penetrations
  • At each change of direction
  • At a minimum of every 20 ft or fraction thereof throughout the piping run.


      A regulator allows the high pressure in a gas cylinder to be reduced to a manageable and usable pressure. A regulator controls delivery pressure and should not be used for flow control. Chose a regulator with an appropriate delivery-pressure range for the application. The maximum should be at least twice as high as the desired pressure.